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| Registros recuperados: 425 | |
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| Le Maréchal, Caroline; Hulin, Olivier; Macé, Sabrina; Chuzeville, Cécile; Rouxel, Sandra; Poëzevara, Typhaine; Mazuet, Christelle; Pozet, Françoise; Sellal, Eric; Martin, Laure; Viry, Alain; Rubbens, Christine; Chemaly, Marianne. |
| We report a botulism outbreak in Charolais cattle fed with wheat flour contaminated by Clostridium botulinum type C and the management of the outbreak at each step from the clinical suspicion to the cleaning and disinfection operations. Diagnosis was based on typical suggestive clinical signs and detection of C. botulinum type C using real-time PCR in samples collected from three young affected bulls. All young exposed bulls and cows (18 animals) eventually died, but three young bulls and one cow were recovering when it was decided to euthanize them. C. botulinum type C was detected in the liver of these four animals. Analysis of the ration components demonstrated that wheat flour, wheat, and the mill used to make flour were positive for C. botulinum type... |
| Tipo: Text |
Palavras-chave: Cattle botulism; Cat carcass; PCR; Wheat; Clostridium botulinum; Feed contamination. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00603/71516/69936.pdf |
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| Cornette, Florence. |
| L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR... |
| Tipo: Text |
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf |
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| Devalle,S.; Niel,C.. |
| Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazil; Co-infections; Multiplex; PCR; Torque teno virus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600006 |
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| Possebon,Fabio Sossai; Costa,Luis Felipe Zuccolo Paschoal da; Yamatogi,Ricardo Seiti; Rodrigues,Marianna Vaz; Sudano,Mateus José; Pinto,José Paes de Almeida Nogueira. |
| A pesquisa de Salmonella em carcaças de aves tem mostrado resultados discrepantes, dependendo se as amostras foram colhidas ainda na indústria, imediatamente após o chiller ou no comércio varejista, quando se encontram submetidas à refrigeração por vários dias. Técnicas mais sensíveis, tais como a Reação em Cadeia pela Polimerase (PCR), podem fornecer dados importantes sobre o efeito do resfriamento sobre as células do patógeno, comparando seus resultados com os da metodologia microbiológica convencional (MC). Foram colhidas 130 carcaças de frango, sendo que 65 foram obtidas ainda na indústria (pós-chiller) e imediatamente analisadas e 65 carcaças embaladas e estocadas a 5°C por 72 horas (simulando o varejo), sendo então realizada a pesquisa do patógeno... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Salmonella; Frango; PCR; Refrigeração. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100021 |
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| Chen,Sique; Zheng,Xiujuan; Cao,Hongrui; Jiang,Linghui; Liu,Fangqian; Sun,Xinli. |
| Background Thermostable DNA polymerase (Taq Pol ?) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. Moreover, we found that 30-40% activity of Taq Pol I was lost at the ammonium sulfate precipitation step, and the product contained a small amount of DNA. Results We provided a novel, simplified and low-cost method to purify the Taq Pol ? after overproduction of the enzyme in Escherichia coli, which used ethanol instead of ammonium sulfate to precipitate the enzyme. The precipitate can be directly dissolved in the storage buffer without dialysis. In addition, DNA and RNA contamination was removed with... |
| Tipo: Journal article |
Palavras-chave: Ethanol precipitation; PCR; Purification; Taq DNA polymerase. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500005 |
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| Ozkan,O; Ahmet,C; Zafer,K. |
| Mitochondrial DNA (mtDNA) has been widely employed in phylogeographic and phylogenetic studies. In the present study, the genetic identification of the scorpion Androctonus crassicauda (Olivier, 1807) was carried out by using the 16S mitochondrial gene, since this scorpion represents the most important species in Turkey regarding scorpionism and antivenom production. Two genetic groups were found according to the sequence analysis results, while five different loci at the nucleotide level presented genetic variation in the 16S region when compared to a known A. crassicauda sequence data (GenBank, AJ277598). Nucleotide variations found in the current work constitute the first descriptive report for A. crassicauda. Moreover, future studies may enlighten the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion; Androctonus crassicauda; PCR; Genotype; Turkey. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400010 |
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| Nogueira,E.O.; Brentano,L.; Ferreira,A.J.P.. |
| A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo virus isolation revealed 100% agreement of the results, with 13 positive and 3 negative samples in both assays, indicating that the VP3/VP1 PCR is a specific diagnostic method. Tissues from additional 24 broiler chicken flocks, with CAV-like lesions and clinical history were then tested only by the VP3/VP1 PCR and a reference PCR with published primers for the VP1 gene. Nineteen samples resulted positive and one negative in both PCR, while another 4 samples were positive only in the VP3/VP1 PCR. These... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Chicken anemia virus; CAV; PCR; Diagnostic test. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800001 |
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| Gouveia,E.M.M.F.; Silva,I.S.; Nakazato,G.; Onselem,V.J.V.; Corrêa,R.A.C.; Araujo,F.R.; Chang,M.R.. |
| The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic E. coli; In vivo experiment; Dogs; Phosphorylated 31 mannanoligo saccharides; PCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200027 |
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| Moo-Koh,F. Amalia; Cristóbal Alejo,J.; Reyes-Ramírez,Arturo; Tun-Suárez,J. María; Sandoval-Luna,Ruth; Ramírez-Pool,J. Abrahán. |
| Las enfermedades causadas por hongos en las plantas disminuyen la calidad y el rendimiento de los cultivos; el control de estas enfermedades demanda investigación enfocada a reducir el uso de los fungicidas sintéticos, los cuales generan resistencia de cepas y contaminación al medio ambiente. El extracto acuoso de Bonellia flammea es una alternativa natural de control ya que actúa como fungicida. El objetivo del presente estudio fue evaluar in vitro el efecto del extracto acuoso de B. flammea contra siete hongos fitopatógenos. El diseño experimental fue completamente al azar, los tratamientos fueron cada hongo expuesto al extracto y el hongo sin extracto, la unidad experimental fueron cuatro cajas petri, con cuatro repeticiones, los datos se analizaron... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inhibición; Control; Fungosis; PCR. |
| Ano: 2014 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000800006 |
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| Yamasaki,L.; Boselli-Grotti,C.C.; Alfieri,A.A.; Silva,E.O.; Oliveira,R.L.; Camargo,P.L.; Bracarense,A.P.F.R.L.. |
| A relação entre Helicobacter spp. e a presença de alterações histológicas na pars esophagea de suínos foi avaliada em 67 estômagos de animais em idade de abate. Para a identificação das helicobactérias, utilizou-se a técnica da PCR com primers específicos para o gênero Helicobacter. As alterações histológicas foram identificadas e classificadas como ulceração, erosão, degeneração epitelial, alongamento de papilas, hiperplasia, paraqueratose, intensidade do infiltrado inflamatório e aumento do número de folículos linfoides. As alterações mais frequentemente encontradas na pars esophagea foram a degeneração epitelial e o alongamento de papilas, observadas em 83,5% (n=56) das amostras analisadas. Em 77,5% (n=52) das amostras, observou-se paraqueratose e em... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Suíno; Estômago; Úlcera gástrica; Helicobacter spp.; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300005 |
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| Vargas,Luciano Kayser; Selbach,Pedro Alberto; Sá,Enílson Luiz Saccol de. |
| A disponibilidade de resíduos de aveia-preta, com relação C:N elevada, resulta em imobilização microbiana de nitrogênio no solo, exigindo cuidados no manejo da adubação nitrogenada da cultura subseqüente. O objetivo deste trabalho foi avaliar as alterações na estrutura da comunidade microbiana ao longo do ciclo do milho, na presença de resíduos de aveia-preta e da aplicação de nitrogênio. Foram coletadas amostras de um Argissolo Vermelho distrófico no dia da semeadura do milho e 46, 62, 88 e 112 dias após a semeadura. O nitrogênio foi aplicado 25 dias e 49 dias após a semeadura. As alterações na comunidade microbiana foram avaliadas mediante relações entre carbono (C) e nitrogênio (N), nitrogênio reativo com ninidrina (N-Nin) e carboidratos (CHO) da... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biomassa microbiana; PCR; Preparos de solo; Imobilização de nitrogênio; Zea mays. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800004 |
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| Sastre,Darío A.; Argaraña,Carlos E.; Heller,Viviana B.; Gallo,Mónica; Fernández,Enrique N.; Rodríguez,Cecilia M.. |
| In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Multiplex-PCR; Ph chromosome; BCR-ABL; Chronic myeloid leukemia; PCR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003 |
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| Registros recuperados: 425 | |
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